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Overview

At the Molecular Biophysics and Biochemistry group of the CMD/TDI Oxford we specialise in chemical probe discovery projects. We carry out assay development, screening campaings and biophysical and biochemical characterisation of hit and lead compounds to determine the thermodynamics of and kinetic parameters of binding. The group also supports and extend the focused libraries of small molecular weight ligands for proteins of interest.

We have supported and carried out or own screening campaings in the fields of

  • kinases
  • epigenetic readers (bromodomains, YEATS domains, spindlins)
  • histone demethylases
  • ADAR family RNA binding proteins
  • NUDIX family proteins

Equipment and capabilities

  • Compound storage and liquid handling:
    • Labcyte Echo 550 in Access workstation
    • Formulatrix Tempest
    • Roylan StoragePopds
  • Multimode plate readers with plate stackers: BMG PherastarFS & FSX
    • HTRF
    • AlphaScreen/AlphaLisa
    • Fluorescence intensity and polarisation
  • Surface Plasmon Resonance: BioacoreS200
  • Biolayer Interferometry: FortéBio OctetRED384
  • Microscale Thermophoresis: Nanotemper Monolith
  • Differential Scanning Fluorimetry:
    • Label free with Nanotemper Prometheus NT.48
    • Dye based thermal shift assays with RT qPCR machines
  • Isothermal Titration Calorimetry:
    • TA Instruments NanoITC
    • Malvern VP-ITC

Our team