Roman Fischer
Associate Professor and Head of Discovery Proteomics Facility
In the Discovery Proteomics Facility of the Target Discovery Institute we provide advice in experimental design, sample preparation, sample analysis with state-of-the-art LCMS workflows and data analysis to researchers from Oxford University and national and international collaborators. We routinely use label-free quantitation, SILAC, TMT, SWATH and other methodologies on diverse samples (i.e. cells, tissues, immuno precipitates et al.) and have developed sample preparation techniques to access the deep proteome form little sample amounts using instrumentation such as Orbitrap Fusion Lumos or TimsTOF Pro.
My own interests evolve around clinical proteomics and applications for the spatial characterisation of the proteome in biological structures such as tissues and tumours. In addition, I am developing methodologies for the proteome characterisation of clinical cohort samples at high-throughput.
Recent publications
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Oncogenic mutations of KRAS modulate its turnover by the CUL3/LZTR1 E3 ligase complex.
Journal article
Damianou A. et al, (2024), Life Sci Alliance, 7
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Dietary n-3 polyunsaturated fatty acids alter the number, fatty acid profile and coagulatory activity of circulating and platelet-derived extracellular vesicles: a randomized, controlled crossover trial.
Journal article
Bozbas E. et al, (2024), Am J Clin Nutr
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A novel method to quantify fibrin-fibrin and fibrin-α2AP cross-links in thrombi formed from human trauma patient plasma.
Journal article
Morrow GB. et al, (2024), J Thromb Haemost
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Quantitative secretomics identifies ligand-receptor pairs in pancreatic cancer that mediate bidirectional crosstalk between cancer stem cells and nociceptors
Preprint
Chang C-H. et al, (2024)
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A systems-based approach to uterine fibroids identifies differential splicing associated with abnormal uterine bleeding
Preprint
Wang CY. et al, (2024)