Roman Fischer
Associate Professor and Head of Discovery Proteomics Facility
In the Discovery Proteomics Facility of the Target Discovery Institute we provide advice in experimental design, sample preparation, sample analysis with state-of-the-art LCMS workflows and data analysis to researchers from Oxford University and national and international collaborators. We routinely use label-free quantitation, SILAC, TMT, SWATH and other methodologies on diverse samples (i.e. cells, tissues, immuno precipitates et al.) and have developed sample preparation techniques to access the deep proteome form little sample amounts using instrumentation such as Orbitrap Fusion Lumos or TimsTOF Pro.
My own interests evolve around clinical proteomics and applications for the spatial characterisation of the proteome in biological structures such as tissues and tumours. In addition, I am developing methodologies for the proteome characterisation of clinical cohort samples at high-throughput.
Recent publications
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T-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles.
Journal article
Céspedes PF. et al, (2022), Nat Commun, 13
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Chronic inflammatory arthritis drives systemic changes in circadian energy metabolism.
Journal article
Downton P. et al, (2022), Proc Natl Acad Sci U S A, 119
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Deep topographic proteomics of a human brain tumour
Davis S. et al, (2022)
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ABPP-HT*-Deep Meets Fast for Activity-Based Profiling of Deubiquitylating Enzymes Using Advanced DIA Mass Spectrometry Methods.
Journal article
Jones HBL. et al, (2022), Int J Mol Sci, 23
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A blood atlas of COVID-19 defines hallmarks of disease severity and specificity
Journal article
Ahern DJ. et al, (2022), Cell, 185, 916 - 938.e58