Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K(+) channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K(+) channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD(+) activate the GsuK channel, whereas Ca(2+) serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K(+) channels.DOI:

Original publication




Journal article



Publication Date





Geobacter sulfurreducens, K+ channel, Other, ligand gating, Adenosine Diphosphate, Allosteric Regulation, Amino Acid Sequence, Bacterial Proteins, Calcium, Crystallography, X-Ray, Escherichia coli, Gene Expression, Geobacter, Ion Channel Gating, Ligand-Gated Ion Channels, Ligands, Models, Molecular, Molecular Sequence Data, NAD, Potassium Channels, Protein Structure, Secondary, Protein Structure, Tertiary, Recombinant Proteins, Sequence Alignment