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The purpose of the study was to analyse how the protein composition of the inflammatory exudate associated with chronic periodontitis differed from the exudate in periodontal health. Gingival crevicular fluid (GCF) was collected from sites with chronic periodontal inflammation and from non-diseased sites in healthy control subjects. Microbore HPLC analysis revealed one major difference in GCF protein profiles between healthy controls and periodontitis patients. The protein enhanced in periodontitis patients was identified as migration inhibitory factor-related protein-8 (MRP-8) by a combination of N-terminal amino acid sequencing, mass spectrometry, and SDS-PAGE. Together, these data demonstrate, for the first time, the presence of monomeric MRP-8 in an inflammatory exudate. Whether monomeric MRP-8 is a unique feature of chronic periodontal inflammation is not yet clear, but the chemotactic properties of this peptide support a functional role for MRP-8 in periodontal inflammation.

Original publication




Journal article


J Pathol

Publication Date





540 - 544


Adult, Amino Acid Sequence, Calcium-Binding Proteins, Calgranulin A, Chromatography, High Pressure Liquid, Chronic Disease, Electrophoresis, Polyacrylamide Gel, Female, Gingival Crevicular Fluid, Humans, Male, Middle Aged, Molecular Sequence Data, Periodontitis, Spectrometry, Mass, Electrospray Ionization