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This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the BacMam system. This eukaryotic expression system was selected and a screening process established in 2016 to enable production of highly challenging human integral membrane proteins (IMPs), which are a significant component of our target list. Here, we discuss our recently developed platform for identifying expression and monodispersity of IMPs from 3 mL of HEK293 cells.

Original publication




Journal article


Methods Mol Biol

Publication Date





95 - 115


BacMam, Baculovirus, FSEC, Gel filtration, IMAC, Insect cells, Mammalian cells, Protein purification, SEC chromatography, Transduction, Gene Expression, Genetic Vectors, HEK293 Cells, Humans, Membrane Proteins, Recombinant Proteins